Adult Lung MKs exhibit significant upregulation of immune markers. (A) Gene-expression analysis of various TLRs, chemokines, and the surface immune marker Cd74 showed a distinct expression pattern with markedly more enrichment for many of these genes in lung MKs. (B) Flow cytometric assessment of TLR2, TLR4, CD74, and MHCII was performed on CD41a⁺/CD42d⁺ MKs. (C) Percentage of cells expressing these immune markers was quantified across 3 replicates, which demonstrated significantly higher expression of TLR2, TLR4, and MHCII in lung MKs. BSA enrichment was not used prior to immune marker analyses. *P < .05.