CIR during FU for patients exceeding the cutoff ≥ 200 NPM1^mut TLs. A total of 75 and 72 patients in remission exceeded the cutoff in ≥1 BM (A) and/or PB (B) sample. The cutoff is highly predictive for relapse in BM (4-year CIR rate, 67.1%; 95% CI: 55.3-78.9) and PB (4-year CIR rate; 71%; 95% CI: 58.9-82.2), with a median time to relapse of 3.8 and 3 months (BM: 95% CI, 0.4-30.8 months; PB: 95% CI, 0.3-32.4 months) determined from the first sample exceeding the cutoff value. Shaded area represents 95% CI.