Sel1l-Hrd1 ERAD is required to maintain HSC pool. Six- to 8-week-old Mx1-cre⁻; Sel1L^fl/fl or Mx1-cre⁻; Sel1L^fl/+ (^+/+), Mx1-cre⁺; Sel1L^fl/+(fl/+) and Mx1-cre⁺; Sel1L^fl/fl (fl/fl) mice were injected with pIpC every other day for a total of 6 doses. Two weeks after pIpC injection, (A) body weight, (B) spleen, and (C) thymus weight, (D) cellularity of bone marrow, (E) spleen and (F) thymus, (G-I) numbers of HSPC, (J,K) lineage restricted progenitors, and (L-M) mature blood cells were analyzed. Data represent mean ± SD. Each replicate represents a single mouse from an independent experiment. Two-sided Student t test was used for statistical analysis.