Sel1l-Hrd1 ERAD is required for HSC reconstitution. Whole bone marrow cells (WBM, 0.3 million) from CD45.2 Mx1-cre⁺; Sel1L^fl/fl (fl/fl) or control (^+/+) mice were transplanted into lethally irradiated CD45.1 mice together with 0.3 million wild-type CD45.1 competitor cells. The contribution of CD45.2 cells in total CD45⁺, myeloid (Mac-1⁺), B (B220⁺), and T (CD3⁺) (A) cells from peripheral blood, and (B) to HSCs and other hematopoietic populations in the bone marrow was analyzed in transplant recipients. Competitive repopulation assay with 50 FACS-purified HSCs from CD45.2 Mx1-cre⁺; Sel1L^fl/fl (fl/fl) or control (^+/+) mice transplanted into irradiated receipt mice along with 0.2 million CD45.1 WBM competitor cells. The contribution of (C) CD45.2 cells in total CD45⁺, myeloid (Mac-1⁺), B (B220⁺), and T (CD3⁺) cells from peripheral blood, and to HSCs and (D) other hematopoietic populations in the bone marrow was analyzed in transplant recipients. Chimerism maintenance analysis with 5 × 10⁵ CD45.2⁺ WBM cells from Mx1-cre⁺; Sel1L^fl/fl (fl/fl) or control (^+/+) mice (without pIpC) transplanted together with 5 × 10⁵ CD45.1⁺ wild-type bone marrow cells into lethally irradiated CD45.1⁺ wild-type recipients. Transplants were injected with pIpC (3 doses) 6 weeks after transplantation. The contribution of (E) CD45.2 cells in total CD45⁺, myeloid (Mac-1⁺), B (B220⁺), and T (CD3⁺) cells from peripheral blood, and (F) in HSCs and other hematopoietic populations in the bone marrow was analyzed in transplant recipients. Data represent mean ± SD. Two to 3 independent experiments were done for each condition and n is described as number of replicates from each experiment, separated by the plus (+) sign. Two-sided Student t test was used for statistical analysis unless specified.