Sequential order of mutation acquisition in t(8;21) AML and inv(16) AML. (A) t(8;21) AML. (B) inv(16) AML. Bradley-Terry plot based on pairwise precedences of gene mutations. Only samples with ≥2 mutations were included in the model. The x-axis reflects the relative order of acquisition. Black dots indicate the point estimate; black lines indicate 95% confidence intervals of gene ordering in time. Genes are positioned along the y-axis on the basis of their relative order of occurrence. The variant allele frequency was adapted for sex and chromosomal aberrations. (C) Schematic illustration of mutational trajectory in CBF-AML leukemogenesis.