Engraftment in runx1 mutants is multilineage and persistent. (A) Experimental schema: donor marrow was harvested from ubi:GFP adult fish and transplanted into runx1-mutant embryos at 2 dpf. Multilineage engraftment was assessed at 2 months (short term) and 6 to 12 months (long term). (B) Dot plot demonstrating the percentages of myeloid (My), erythroid (Ery), lymphoid (Ly), and precursor (Pre) cells that are GFP⁺ in 15 different ubi:GFP donor marrows. The vast majority of myeloid cells are GFP⁺, but there is much more variable GFP expression in the other lineages. (C-F) Myeloid (C), erythroid (D), lymphoid (E), and precursor (F) chimerism values for individual transplanted runx1-mutant fish, as assessed by percent GFP⁺ cells within each lineage at 2 months (short term) or 6 to 12 months (long term) posttransplant. The yellow line indicates 5% chimerism cutoff for engraftment. Comparison between the 2 groups is not significant by Mann-Whitney U test for any of the 4 blood lineages. (G) Lineage distribution of donor-derived GFP⁺ cells in individual engrafted runx1-mutant fish at 2 months (short term) or 6 to 12 months (long term) posttransplant, as well as in representative ubi:GFP donors. (H) The percentage of GFP⁺ donor-derived cells from the myeloid lineage in transplanted recipients at short-term (ST) and long-term (LT) time points posttransplantation. Error bars are standard deviation. Statistical analysis was performed via Mann-Whitney U test. *P < .05. NS, not significant.