Affinity of ciraparantag for binding to heparin in the presence of NaCl at different concentrations. Ciraparantag (CIRA) was eluted from the heparin-coated column at a later time point compared with the acetate control (7.10 vs 2.47 minutes, respectively), when at 0.55 M sodium chloride. An increase in sodium chloride concentration from 0.55 M to 0.75 M led to a gradual decrease in the elution time of ciraparantag to 4.39 minutes at 0.75 M.