An important role for the TRIB1/ERG axis in human AML. (A) Quantitative RT-PCR for TRIB1, HOXA9, and ERG in human AML cell lines. (B) Downregulation of TRIB1/HOXA9 downstream genes in KU812 cells by TRIB1 silencing. Relative gene expression to shControl in KU812 cells. (C) Growth inhibition by TRIB1 knockdown. Cell numbers of KU812 cells treated with 2 different shRNA lentivirus. (D) KU812 and P39 cells were treated with JQ1 at the indicated dosages for 3 days. Cell numbers at the indicated time points are shown. (E) Effects of JQ1 on gene expression in KU812 and P39 cells. Relative mRNA expression of the indicated genes is shown. (F) Kaplan-Meier survival curves for patients for patients with normal-karyotype AML⁶⁴  between high and low expression of ERG (left) or SPNS2 (right). Significances were measured by a log-rank test. Hazard ratios (HRs) and 95% confidence intervals (CIs) are indicated. *P < .05, **P < .01, ***P < .001.