Figure 2.
Platelet-derived plasminogen is retained in a distinct pattern on activated platelets. Platelets (0.5 × 108 platelets/mL) were stimulated on a collagen (0.6 µg)- and thrombin (3 pmol)-coated surface stained with (A) Annexin V-AF488 and anti-plasminogen antibody DL633. Arrow indicates plasminogen in the platelet cap. Arrowheads indicate plasminogen over the granulomere. (B) Time course of platelet activation. Platelets are labeled with Annexin V-AF568, AF488 P-selectin antibody, and anti-plasminogen antibody DL633. Arrowheads indicate the single platelets at different time points. Scale bars, 5 μm; representative images of n ≥ 3.