Systematical screening of chromatin remodelers controlling fetal HSPC development. (A) Scatter plots showing the expression level of chromatin remodeling related genes in nascent and fetal HSPCs based on RNA-seq. Differentially expressed genes detected by DESeq2 were labeled. (B) SOM analysis for RNA-seq data in nascent and fetal HSPCs. (C) Expression of chd2, chd4b, smarca2, smarca4a, bptf, smarca5, and ino80 in nascent and fetal HSPCs by quantitative polymerase chain reaction (qPCR) analysis. The expression level of chd2 in nascent HSPCs was set at 1. (D) Expression of cmyb in the CHT region in control embryos and chd2, chd4b, smarca2, smarca4a, bptf, smarca5, and ino80 morphants at 3 dpf and 4 dpf by WISH. The quantification of WISH results is shown (right). Scale bars, 100 μm. Data are means ± SD (C, D). Asterisk presents statistical significance (*P < .05, **P < .01, ***P < .001). P values were calculated by a 2-tailed, unpaired Student t test. FPKM, fragments per kilobase million; mRNA, messenger RNA.