Deletion of smarca5 leads to the decrease of chromatin accessibility. (A) Heat maps showing ATAC-seq signals around ATAC-seq peaks in sibling and mutant at promoters and distal regulatory regions. Peaks were classified into sibling-specific accessible, both accessible, and mutant-specific accessible. In each group, peaks are ranked according to ATAC-seq signals. (B) GO analysis of genes with accessible promoter regions in HSPCs in smarca5^zko1049a and their siblings. (C) Box plot showing the log₂-transformed fold change of ATAC-seq signals at fetal HSPC-specific and both accessible regions at promoters and distal regulatory regions after smarca5 knockout (KO). smarca5 KO leads to significant decrease of ATAC-seq signals especially at both accessible promoter regions. P values were calculated by a 2-tailed Wilcoxon test. (D) Pie charts drew by CEAS⁵⁶  showing the distribution of Smarca5-ChIP-seq peaks (top) and genome distribution background (bottom) across the genome. (E) The genome browser views showing ATAC-seq signals of genes with (top) or without (bottom) Smarca5 binding. (F) Bar plot showing the proportion of Smarca5 binding sites distributed on both accessible chromatin regions in nascent and fetal HSPCs, specific accessible regions in nascent or fetal HSPCs compared with background (total). UTR, untranslated region.