Ncl collaborates with Smarca5 to facilitate chromatin remodeling. (A) Analysis of the interaction proteins with Smarca5 using MS. Heat maps showing the number of peptide spectrum match (PSM) identified by MS for proteins after immunoprecipitation of Flag-Smarca5 from the CHT region of zebrafish embryos at 3 dpf. (B) Double-fluorescence in situ hybridization analysis showing the coexpression between ybx1, ncl with smarca5 in CHT. Scale bars, 50 μm. (C) Zebrafish embryos were injected with the indicated plasmids at 1-cell stage and CoIP was performed at 3 dpf, immunoblot analysis with the indicated antibodies showing the interaction between Ybx1, Ncl, and Smarca5. (D) Expression of runx1, cmyb, gata1, and rag1 in the AGM, CHT, or thymus regions in ncl^m4 and their siblings from 36 hpf to 4 dpf by WISH. The quantification of WISH results is shown (right). Scale bars, 100 μm. (E) Box plot showing the log₂-transformed fold change of ATAC-seq signals at after smarca5 knockout at Smarca5 only, cobinding, and Ncl only regions. P values were calculated by a 2-tailed Wilcoxon test. Data are mean ± SD. (D) Asterisk presents statistical significance (***P < .001; n.s., not significant). P values were calculated by a 2-tailed, unpaired Student t test.