Plasminogen activator inhibitor 1 and fibrinolytic factors expression in human pancreatic cancer cell lines. We analyzed PAI-1 expression in 5 human pancreatic cancer cell lines. (A) Levels of PAI-1 mRNA in the different cell lines were from the Cancer Cell Line Encyclopedia database. The y-axis represents reads per kilobase of transcript per million mapped reads (RPKM) of RNA sequencing data. Data are shown as median ± interquartile range. (B) Levels of total PAI-1 in culture supernatant were determined by a commercial ELISA (n = 4). (C) Levels of tPA (top), uPA (middle), and uPAR (bottom) mRNA were from the Cancer Cell Line Encyclopedia database.