Proteomic comparison of control and GPS CD34⁺HSC-derived neutrophil culture. (A) Representative plots of the 3 isolated cell populations obtained by fluorescence-activated cell sorting based on cell surface markers CD11b and CD16 from control or GPS CD34⁺ HSC-derived cells at day 10 or 17 of the cell culture. (B) Stacked bar plots showing proportions of measured granule protein content between control (CTRL) PBMC CD34⁺ HSCs cultured CD16^POS vs blood-derived PMNs (left panel) and GPS PBMC CD34⁺ HSCs cultured CD16^POS vs blood-derived PMNs (right panel). (C) Comparisons among proteomes of the 3 different development stages (CD11b^NEGCD16^NEG, CD11b^POSCD16^NEG, and CD11b^POSCD16^POS) at the end of differentiation from the CD34⁺ HSC cultures derived from healthy controls or patients with GPS.