KPT-330+CS is a better inhibitor of nuclear export and decreases CRM1 expression. (A) Immunofluorescence microscopy images obtained from Zeiss LSM 780 confocal microscope at original magnification ×60 showing the subcellular localization of GFP (green) and endogenous CRM1 (red) in U2OS cells transfected with nuclear export reporter construct (NLS-GFP-NES), and treated with the indicated conditions for 24 hours. (B) Quantitation of nuclear export efficiency. GFP-transfected U2OS cells were evaluated and scored for percentage with complete nuclear localization of GFP signal. (C) Immunoblot images showing the expression of transfected CRM1-YFP and endogenous CRM1 in U2OS, HeLa, and HEK293 cells treated with the indicated conditions for 24 hours. *P = .02. The paired Student t test was used to compare all continuous variables. A value of P < .05 was considered statistically significant.