Transfusion of KEL^lo, KEL^med, and KEL^hiRBCs into anti-KEL antibody immunized recipients results in antigen modulation. (A-D) RBCs with KEL^lo (green), KEL^med (blue), and KEL^hi (red) were monitored posttransfusion for IgG deposition over time by flow cytometry at 10 minutes, and 1, 2, 4, 6, and 24 hours posttransfusion into PBS-treated control recipients (A) or recipients that possessed low (B), medium (med) (C), or high (hi) (D) αKEL levels. (E-H) RBCs with KEL^lo (green), KEL^med (blue), and KEL^hi (red) were examined for total KEL antigen detected by flow cytometry at 10 minutes, and 1, 2, 4, 6, and 24 hours posttransfusion into PBS-treated (E) control recipients or recipients with low (F), medium (med) (G), or high (hi) (H) αKEL levels. Error bars represent mean ± SEM. n = 5-7 mice per group. Each panel represents data reproduced 3 times. *Significance indicated vs value at 10 minutes posttransfusion. P < .05.