Anti-KEL antibodies induce changes in KEL antigen levels independent of KEL RBC clearance. (A) KEL^lo (green), KEL^med (blue), and KEL^hi (red) RBC survival was plotted against KEL antigen levels relative to nonimmunized recipients at 10 minutes, and 1, 2, 4, 6, and 24 hours posttransfusion in recipients immunized with 2 µL anti-KEL antibodies (low αKEL). (B) KEL^lo (green), KEL^med (blue), and KEL^hi (red) RBC survival was plotted against KEL antigen levels relative to nonimmunized recipients at 10 minutes, and 1, 2, 4, 6, and 24 hours posttransfusion in recipients immunized with 6.25 µL anti-KEL antibodies (med αKEL). (C) KEL^lo (green), KEL^med (blue), and KEL^hi (red) RBC survival was plotted against KEL antigen levels relative to nonimmunized recipients at 10 minutes, and 1, 2, 4, 6, and 24 hours posttransfusion in recipients immunized with 24 µL anti-KEL antibodies (hi αKEL). (D-G) Ter119 levels on KEL^lo (green), KEL^med (blue), and KEL^hi (red) were evaluated at 10 minutes, and 1, 2, 4, 6, and 24 hours posttransfusion by flow cytometric examination. Error bars represent mean ± SEM. n = 5-7 mice per group. Each panel represents data reproduced 3 times.