In vivo microthrombi examination in a DIC rodent model. (A) Shown are LPS-induced DIC rodent model harvested organs, including heart, lungs, kidneys, and liver, with corresponding photos of whole organs, MSB-stained tissue sections, and IHC for fibrin deposition in tissue sections. (B-E) DIC animals were treated with saline (n = 5), unloaded FSNs (n = 5), tPA-FSNs (n = 5), and tPA-CS-IgG (n = 5) and compared with controls (n = 6). Images were taken on an EVOS FL Auto Imaging System at 10× magnification. Corresponding quantification (performed with ImageJ particle analysis software) for IHC of fibrin deposition in heart (B), lung (C), kidney (D), and liver (E) tissue sections was averaged using 3 images per section for each animal (n = 6 for control liver, n = 4 for DIC + tPA-FSN lung, and n = 5 for all other groups). Data are presented as average ± standard deviation. Data sets were analyzed via 1-way ANOVA with a Tukey’s post hoc test using a 95% confidence interval. DAPI, 4′,6-diamidino-2-phenylindole. *P < .05; **P < .01; ***P < .001; ****P < .0001.