Description of AK2-depleted T-ALL blast apoptosis. (A-C) Mitochondrial depolarization was analyzed by using MitoProbe DilC (5) reagent in JURKAT (A), RPMI 8402 (B), and REH (C) cells transduced with shScramble (gray) or shAK2 (red) with a positive control of depolarization in the presence of carbonyl cyanide 3-chlorophenylhydrazone (CCCP) (green). (D-F) ROS levels in JURKAT (D), RPMI 8402 (E), and REH (F) cell lines transduced with shScramble (gray) or shAK2 (red) detected by using the CellROX probe, in the presence of a positive control of ROS production (hydrogen peroxide [H₂O₂]; green) and a positive control of inhibitor of ROS (NAC; blue). (G) Apoptosis array showing relative levels of the main proteins upregulated (red) or downregulated (blue) implicated in the apoptotic process in the JURKAT cell line transduced by shAK2 or shScramble at day 3 posttransduction. All experiments were performed in technical duplicates.