Figure 2.
Effect of drugs inhibiting RUNX1 proteolytic degradation on RUNX1 protein and transcript levels in 293T cells transiently transfected with RUNX1-FLAG. (A) RUNX1 protein degradation pathway, showing the points at which inhibitors act. (B) Western blot analysis exhibiting elevated levels of exogenous RUNX1-FLAG protein expression in transiently transfected 293T cells after 24 hours of drug treatment vs vehicle (DMSO) treatment. (C) qRT-PCR revealing commensurately elevated levels of RUNX1 transcript in drug-treated 293T cells transiently transfected with RUNX1-FLAG. Error bars show standard deviation of 2 repetitions. Drug concentrations for all experiments are listed in supplemental Table 1. **P < .01, using a 2-tailed Student t test assuming unequal variance.

Effect of drugs inhibiting RUNX1 proteolytic degradation on RUNX1 protein and transcript levels in 293T cells transiently transfected with RUNX1-FLAG. (A) RUNX1 protein degradation pathway, showing the points at which inhibitors act. (B) Western blot analysis exhibiting elevated levels of exogenous RUNX1-FLAG protein expression in transiently transfected 293T cells after 24 hours of drug treatment vs vehicle (DMSO) treatment. (C) qRT-PCR revealing commensurately elevated levels of RUNX1 transcript in drug-treated 293T cells transiently transfected with RUNX1-FLAG. Error bars show standard deviation of 2 repetitions. Drug concentrations for all experiments are listed in supplemental Table 1. **P < .01, using a 2-tailed Student t test assuming unequal variance.

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