B-cell–specific constitutive activation of Akt in the Eµ-TCL1 mouse model induces aggressive lymphoma with a diffuse large B-cell phenotype in vivo. (A) Breeding scheme required to generate requisite genotypes. (B) Relative quantification of Cd5⁺ B cells from serial blood samples from Cd19-Cre (black), Cd19-Cre^Akt-C (gray), Eµ-TCL1 (blue), and Eµ-TCL1^Akt-C (red) mice. Blood samples were measured between 2 months and 10 months of age with 1-month intervals. (C) Pairwise Kaplan-Meier overall survival analysis. Significance between groups was tested by using the pairwise log-rank P values. (D) Mean cell size (FSC value) of Cd5⁺ B cells from peripheral blood measured between 2 and 10 months of age with 1-month intervals. (E) Representative hematoxylin and eosin staining of blood samples. (F) Representative images of spleens from Cd19-Cre (black; top left), Cd19-Cre^Akt-C (gray; bottom left), Eµ-TCL1 (blue; top right), and Eµ-TCL1^Akt-C (red; bottom right) mice aged 3 to 4 months and 7 to 8 months, respectively. (G) Box plot of spleen weight for mice aged 3 to 4 months and 7 to 8 months. (H) Box plot showing the mean cell size (FSC value) within the spleen for mice aged 3 to 4 months and 7 to 8 months. (I) Representative hematoxylin and eosin staining of spleen preparations. (J) Bar graph displaying relative levels of B cells showing low, medium, and high amounts of Ki-67 in mice aged 3 to 4 months and 7 to 8 months. (K) Box plot showing the lactate dehydrogenase (LDH) levels for mice aged 3 to 4 months and 7 to 8 months. *P < .05, **P ≤ .01, ***P ≤ .001 (unpaired, 2-sided Student t test).