Overexpression of S100a4, increased phosphorylation of Hes1, and increased activation of Cdk4 and Wee1 infer Notch1 activation. (A) Flow diagram of phosphoproteomic/proteomic experimental setup. (B) Log₂ label-free quantification (LFQ) intensity values of all proteins captured (left panel) or phosphopeptides (right panel) across all mice per genotype. (C) Volcano plots representing the difference in log₂ LFQ intensity of proteins (left panel) and phosphopeptides (right panel) between Eµ-TCL1 (n = 4) and Eµ-TCL1^Akt-C (n = 4) mice. Significant DE proteins/phosphopeptides defined as difference >1 and P < .05 (upregulated in Eµ-TCL1^Akt-C = yellow; downregulated = pink). (D) Normalized upstream kinase scores for all 45 kinases with available PhosphoSitePlus kinome data. Bars represent the difference in upstream kinase scores between Eµ-TCL1 (n = 4) and Eµ-TCL1^Akt-C (n = 4) (upregulated in Eµ-TCL1^Akt-C = yellow; downregulated = pink). (E) Upstream kinase scores from the top significantly upregulated kinases in Eµ-TCL1^Akt-C mice. Significant differences between groups assessed by using Student t test, significant proteins/peptides defined as P < .05 as well as difference >1 /<1 for phosphoproteomics/proteomics, whereas for upstream kinase analysis. P < .05 kinases were considered significant. NS, not significant.