AnVWF shows conserved multimerization and human ADAMTS13 proteolysis. Purified recombinant AnVWF was analyzed for multimer formation using sodium dodecyl sulfate–agarose electrophoresis following incubation in the absence (A) or presence (B) of recombinant human ADAMTS13. (C) Percentage of VWF multimer cleavage was determined by densitometry before and after enzyme addition and normalized to background signal.