PML activates metabolic pathways in ELANE-I60F HPCs. (A) GSEA comparing PML proficient and deficient cells showing that PML induces MYC and mTORC1 signaling in ELANE-I60F, but not in control HPCs. Data are derived from 3 independent experiments. (B) Oxygen consumption rate (OCR) measured with the Seahorse mitochondrial stress test (Agilent) showing PML-dependent increases in basal respiration, ATP, and maximum respiration in ELANE-I60F cells (n = 5). FDR, false discovery rate; NES, normalized enrichment score. ***P < .001, ****P < .0001.