Grna inhibits gata1 expression. (A) RNA-seq analysis from grna^−/− and grna^+/+ adult zebrafish kidney marrows reveals 154 downregulated and 116 upregulated genes in grna^−/− vs grna^+/+ control. (B) Volcano plot obtained from DESeq2 analysis of grna^−/− and grna^+/+ kidney marrows. (C) Heat map of the enriched and depleted transcripts in kidney marrows from grna^−/− vs grna^+/+ adult fish. Color coding is based on rlog-transformed read count values. (D) Representative fluorescence images of 48 hpf Gata1:DsRed embryos injected with Grna-MO (bottom panel) and a 5-base Grna mismatch control (upper panel). (E) Quantification by flow cytometry showing the histogram of 3 pooled Gata1:DsRed embryos injected with Grna-MO (red) or mismatch Grna-MO control (gray). (F-G) Erythrocyte numbers quantified by flow cytometry of Gata1:DsRed (F) or LCR:eGFP (G) embryos injected with Grna-MO or Grna mismatch control MO. Dots represent independent biological replicates from 3 48-hpf Gata1:DsRed⁺ pooled embryos (F) or 3 48-hpf LCR:eGFP⁺ pooled embryos (G). (H) Hematocrit (%) in grna^+/+ and grna^−/− adult zebrafish. Horizontal lines indicate mean ± SEM. MUT, mutated; WT, wild-type.