Macrophages respond abnormally to injury in the absence of Grna. (A) Experimental workflow. Tg(Mpeg1:eGFP) 1-cell stage embryos were injected with either Grna MO or Grna mismatch MO. At 48 hpf, caudal tails were resected immediately after the end of the notochord. Fluorescence imaging of the tail (CHT, where the majority of neutrophils reside at this developmental time, and wound area) was taken every 2 hours from 1 hour post-wounding (hpw) to 32 hpw, and the number of neutrophils was quantified manually. (B) Neutrophil numbers in the CHT from individual Mpeg1:eGFP transgenic animals at 1, 3, 5, 7, and 9 hpw after depletion of Grna compared with Grna mismatch control morphants. (C) Percentage of Mpeg1:eGFP⁺ macrophages recruited to the wound region normalized to the total macrophage number in the tail (CHT and wound) in embryos injected with Grna MO (red line, n = 3) or Grna mismatch MO (blue line, n = 3) at indicated time points. Circle and square dots indicate means, and error bars indicate SEM. (D) Representative fluorescence images of tail fins from Mpeg1:eGFP Grna or Grna mismatch control morphant siblings at the indicated times. *P < .05; **P < .001.