Single-cell genomic analysis of T-ALL. Genomic DNA from single T-ALL cells are isolated and barcoded using the Mission Bio Tapestri platform. Alleles are sequenced by NGS using a bespoke 305-amplicon panel for 110 genes on PCR-amplified barcoded genomic DNA. Informative alleles are identified after sample and amplicon filtering, identification of allele dropouts (ADOs), and variant curation. T-ALL single-cell clonal architecture is reconstructed. BM, bone marrow; PB, peripheral blood; WT, wild type.