PD profile of rhFVIII with or without VWF-12. F8^−/− mice received a single dose of IV rhFVIII (200 IU/kg) alone, subcutaneous rhFVIII/VWF-12 (1000 IU/kg), or no treatment (Ctrl.) Time in hours from treatment to bleeding challenge is indicated. Hemoglobin loss is shown over 30 minutes from tail clipping with higher values indicating more bleeding. Boxes show median and interquartile ranges; whiskers show the 10th and 90th percentiles. Number of mice per group is shown in the top line. Outliers were determined using the ROUT procedure and are represented by dots.