Outcome and immune reconstitution of patients enrolled in the study. Cumulative incidence of (A) aGVHD and (B) cGVHD in the study population. Probability of (C) EFS and (D) OS. (E) Reconstitution kinetics of αβ⁺, γδ⁺ T cells and NK cells in patients receiving TCRαβ⁺/CD19⁺-depleted haplo-HSCT. A rapid recovery of γδ⁺ T and NK cells was observed within the first few weeks posttransplant followed by a progressive increase of αβ⁺ T and B lymphocytes over time. Reconstitution kinetics of (F) CD4⁺ and CD8⁺ T cells and (G) B lymphocytes. (H) Kinetics of immunoglobulin serum level recovery: median (dashed thick line), 25th and 75th percentiles (dotted thin lines), and ranges are represented in violin plots. Immunoglobulin recovery was observed in parallel to B-cell reconstitution. Peripheral blood samples were obtained 1, 3, 6, and 12 months after HSCT. Absolute numbers of each cell subset are shown together with standard error of the mean. E, events; LNN, lower limit of normal; N, number; yr, year.