Reversal of thrombocytopenia with the restoration of TPO production and organomegaly in infected mice after treatment.L donovani–infected mice were treated with a single IV dose of AmBisome (8 mg/kg) at day 28 PI and bled weekly via tail vein for platelet counts. Platelet counts (A), MPV (B), and postmortem liver weights (C) of AmBisome-treated and uninfected control mice. Data are representative of a single experiment and were analyzed with the unpaired Student t test comparing means ± SD of treated vs uninfected control at each time point. (D) Circulating TPO levels were measured weekly in serum samples of uninfected control (n = 5) and treated (n = 3 at weeks 1, 2, 3, and 4) mice. Data are representative of a single experiment and analyzed using ANOVA with the post hoc Tukey’s test. (E) Hepatic Thpo mRNA accumulation was measured using quantitative RT-PCR on postmortem liver samples of uninfected control (n = 3) and treated (n = 3) mice at each time point. Data are representative of a single experiment and analyzed using the unpaired Student t test comparing the mean ± SD of control vs treated mice. (F) Postmortem spleen weights (g) of AmBisome-treated and uninfected control mice. Data are representative of a single experiment and analyzed using the unpaired Student t test comparing the mean ± SD of treated vs uninfected control at each time point. *P < .05; **P < .01; ***P < .001; ****P < .0001.