Clot Treg profile. CD69⁺(Foxp3)-GFP⁺ Tregs were sorted from day 14 thrombi, vein walls, and spleens (n = 4 independent preparations) of DEREG mice and subjected to RNA sequencing. Box-whisker plots of reads per kilobase million (RPKM) values are shown for canonical Treg signature genes (A), traditional suppressive molecules (B), Treg effector program and TCR/cytokine signaling genes (C), and tissue Treg genes (D). Mean values ± SEM (*P ≤ .05; **P ≤ .005) are shown. All statistical differences were tested using an unpaired Student t test (2 tailed).