SPARC induces MMP activity in intrathrombic monocytes. (A) Sorted day 14 clot Tregs were stimulated with plate-bound anti-CD3 mAb or recombinant cytokines for 24 hours, and SPARC levels were determined in culture supernatant by ELISA. (B) Unseparated day 14 thrombi and vein samples were incubated for 24 hours in the presence of MMPSense with or without 50 ng/mL recombinant murine SPARC (rSPARC) protein. Representative flow cytometric plots of CD45⁺Lin⁻CD11b⁺ monocytes shown. Statistical differences were tested using an unpaired Student t test (2 tailed). Mean values ± SEM are shown (**P ≤ .005; ***P ≤ .0005; ns, not significant). Results are representative of 2 independent experiments. w/o, without.