Therapeutic Treg expansion window. (A) Size development of thrombi in B6 mice injected with IL-2/JES 6-1 complexes at days 2 to 5 after IVC ligation. (B) Nonregulatory CD25⁺ T-cell frequencies in thrombi and blood at day 2, (n = 5 group). (C) Size development of thrombi in B6 mice injected with IL-2/JES 6-1 complexes at days 8 and 12 after primary IVC ligation and religated upon complete resolution of the primary thrombus. Mean results ± SEM are shown (n = 3; **P ≤ .005; ***P ≤ .0005). Statistical differences were tested using an unpaired Student t test (2 tailed).