Analysis of GFI1 targets and their association with PML/RARα targets. (A) Heatmap showing the ChIP-seq signals of PML/RARα and GFI1 centering on the binding summit of PML/RARα and GFI1 cobound peaks. (B) Motif analysis of peaks cobound by PML/RARα and GFI1. Top 6 enriched motifs were shown. (C) Genome-browser tracks showing the binding of PML/RARα, GFI1, RUNX1, and FLI1 on the regulatory regions of CXCR4 and MPO in NB4 cells. (D) Gene Ontology enrichment analysis showing Biological Process terms enriched on PML/RARα and GFI1 coregulated genes. (E) GSEA of PML/RARα and GFI1 coregulated genes in terms of differential genes upon shRNA-mediated knockdown of GFI1 (indicated by graded bars). Also labeled are representative genes found at the leading edge. NES, normalized enrichment score. The asterisk indicates that GFI1 downregulation was due to knockdown using shGFI1. (F) Knockdown GFI1 decreased PML/RARα binding on chromatin of GFI1 and PML/RARα cobound and coactivated genes MPO and CXCR4. ChIP-qPCR of PML/RARα binding was performed in NB4 cells with or without GFI1 knockdown. The fold enrichment of ChIPed samples vs input was plotted in the y-axis. *P < .01, **P < .001