PD1⁺TIM3⁺CD4⁺T cells and PD1⁺TIM3⁺CD8⁺T cells are enriched in ABC DLBCL tissues. (A) Representative histograms of PD1, PD-L1, PD-L2, TIM3, and CD80 expression on CD8⁺ T cells from 1 rLN and 1 DLBCL sample (left). PD1, PD-L1, PD-L2, BTLA, HVEM, CD80, TIM3, HLA-DR, and Ki67 expression on CD4⁺ and CD8⁺ T cells in DLBCL (n = 25), FL (n = 15), cHL (n = 10), and rLN (n = 7) samples (right). The percentage of Ki67⁺ cells and median fluorescence intensity (MFI) for other markers were normalized and hierarchically clustered using Tmev software. (B) Percentage of CD4⁺ and CD8⁺ T cells expressing PD1 and TIM3 in DLBCL (n = 25), FL (n = 15), cHL (n = 10), and rLN (n = 7) samples. (C) Percentage of PD1⁺TIM3⁺ CD8⁺ T cells and PD1⁺TIM3⁺ CD4⁺ T cells in GC DLBCL (n = 9), ABC DLBCL (n = 14), and FL (n = 15) samples. Correlation between percentages of PD1⁺TIM3⁺ CD8⁺ T cells and percentages of PD1⁺TIM3⁺ CD4⁺ T cells on DLBCL samples (GC, blue; ABC, red; unknown, green). (D) Representative mIHC staining in a DLBCL sample (magnification ×100). Yellow arrows show a CD8⁺ (blue), PD1⁺ (red), TIM3⁺ (green) cell that expresses Ki67 (white). *P < .05, **P < .01, ****P < .0001 by Mann-Whitney nonparametric U test.