CD8⁺T-cell expression of PD1 and TIM3 in DLBCL vs rLN and their colocalization with B cells. In this DLBCL sample, the yellow box shows 4 cells expressing CD8 (blue), PD1 (red), and TIM3 (green) in contact with CD20⁺ lymphoma cells, identified by the white box. These CD8⁺ T cells frequently localize in the CD20⁺ infiltrate (turquoise). In this rLN sample, the CD8⁺ T cells (blue) sometimes express PD1 (red) but not TIM3 (green). They are found at the periphery of the CD20⁺ cluster. Original magnification ×60.