Quantification of GC lymphocytes at day 10 after immunization with SRBCs. (A) Representative fluorescence-activated cell sorting analysis of splenic lymphocytes stained for CD95 and GL7 from a representative Rosa26^HGAL/Aid-Cre mouse (upper panels) and a control Rosa26^HGAL mouse (lower panels). (B) Bar graph showing the percentage of GC lymphocytes in the gated B220⁺ population from Rosa26^HGAL mice, Rosa26^HGAL/Aid-Cre mice, Rosa26^HGAL/Mb1-Cre mice, Rosa26^HGAL/Sca1-Cre mice, and control WT mice (n = 6 each) (left panel). Line graph showing GFP intensity in GC lymphocytes in each genotype (right panel). *P < .05; **P < .01, Bonferroni multiple-comparison test (1-way analysis of variance).