VPS45 controls the intracellular organization of endolysosomal vesicles. (A) Immunoblot analysis of expression levels of VPS45 binding partners Rabenosyn-5 and Syntaxin 16 in control and VPS45 KO HeLa clones (n = 3 different clones per genotype). One representative experiment of 2 independent experiments is shown. (B) Immunofluorescence microscopy analysis of control and VPS45 KO HeLa clones to visualize early endosomes (EEA1), late endosomes (LAMP2), lysosomes (LAMP1), and the nucleus (4′,6-diamidino-2-phenylindole [DAPI]). One representative experiment of 3 independent experiments is shown. Scale bars, 10 µm. (C) Immunoblot analysis of expression levels of EEA1, LAMP2, and LAMP1 in control and VPS45 KO HeLa clones (n = 3 different clones per genotype). One representative experiment of 2 independent experiments is shown. ACTB, β-actin; Ctrl, control.