Renal damage and MET formation are reduced in DKO mice. Phenotypic analyses of RAKI WT and CLEC-2–depleted FcRγ-deficient (DKO) mice. (A) Representative macroscopic images of kidneys showing RAKI-induced ischemia. The top panel indicates water-injected (non-rhabdomyolytic) WT kidneys. (B) Serum creatinine (Cre) level at 48 hours after glycerol injection into WT and DKO mice. Data are expressed as mean ± standard deviation; n = 11 and 9, respectively. (C) Renal tubular injury evaluated by periodic acid–Schiff staining at 48 hours after glycerol injection into WT and DKO mice. Scale bars: 50 μm. Data are expressed as mean ± standard deviation; n = 7. (D) Immunofluorescence imaging of citrullinated histone H3 (CitH3, red), F4/80 (green), and 4′,6-diamidino-2-phenylindole (DAPI) (blue) for the detection of MET formation in renal tubules at 8 hours after glycerol injection into WT and DKO mice. The arrow indicates macrophages undergoing MET formation. Magnified single-color views of a framed area in each merged image are shown on the right.  Scale bars: 50 μm. Data are expressed as mean ± standard deviation; n = 6. *P < .05, **P < .01.