MET formation by hemin-activated platelets requires activation of the SFK pathway in platelets. (A) Macrophages differentiated from THP-1 were stimulated by resting platelets (b), hemin (c), hemin-activated platelets (d), hemin-activated platelets with PP2 (e), PMA (f), and PMA with PP2 (g). Representative images of SYTOX Green (green) and Hoechst 33342 (blue) staining are shown. Arrows indicate MET-like structures. Scale bars: 50 μm. (B) Quantification of MET-like structures. Data in the bar graph are expressed as mean ± standard error of the mean; n = 4. *P < .05, **P < .01. Holm-Šidák multiple-comparison test was used. ns, not significant.