T-cell differentiation markers are enhanced in the spleen after ABT-199 treatment. Volcano plot representations of the DE genes for LOUCY cells collected from the spleen (A) and bone marrow (B) after vehicle and ABT-199 treatment. Red and blue points mark the genes with significantly increased or decreased expression, respectively. The x-axis shows log fold changes in expression and the y-axis the log odds of a DE gene. The P values were determined by using the Wilcox rank sum test with adjusted P values calculated using Bonferroni correction in Seurat. (C) Enriched Gene Ontology (GO) terms of DE genes in the spleen (clusters 3, 6, 12, and 15) after ABT-199 treatment. The 10 most significantly (P < .05) enriched GO terms in molecular functions and biological processes are presented. All the adjusted statistically significant values of the terms were negative 10-base log transformed. The P values were determined by using Enrichr with default parameters. (D) GO functional analysis of DE genes in the bone marrow (clusters 4, 5, and 15) after ABT-199 treatment. (E) cMYB gene scatterplot in LOUCY cells from the spleen of vehicle- and ABT-199–treated mice. (F) GATA3 gene scatterplot in LOUCY cells from the spleen of vehicle- and ABT-199–treated mice. (G-H) cMYB and GATA3 expression from a T-ALL patient cohort of 264 primary samples using RNA-Seq data set.²⁸  One-way analysis of variance with Tukey’s multiple comparison post hoc test for statistical significance values. **P < .01, ****P < .001.