MYC-N11S polymorphism is associated with decreased MYC protein expression as detected by IHC using the Y69 antibody. (A) MYC mRNA expression is shown in the bottom track as a heatmap for the 446 tumors in which MYC sequencing data were available. Tumors are linearly arranged from lowest to highest expression, and the corresponding N11S status, MYC IHC status, and MYC rearrangement status for each tumor are shown in the tracks above the heatmap. Break-apart positive (BA POS) refers to tumors positive for MYC rearrangement (MYC^Tr). (B) Expression of MYC mRNA in N11S-positive (POS) and N11S-negative (NEG) tumors. P value was determined by an unpaired 2-sided Student t test. Error bars represent the mean and 95% confidence intervals. (C) Percentage of tumor cells positive for MYC IHC staining in N11S POS and NEG tumors. The red dotted line indicates the ≥40% threshold used to identify MYC IHC positivity. The P value was determined by using the Mann-Whitney U test. Only tumors with IHC scores on a 10% increment scale of percent positively stained cells are included in B and C (n = 323).