Adhesion of HNA-3a–expressing neutrophils to VWF is amplified by LPS and does not require β₂ integrins. (A) Percentage of total tracked neutrophils qualified as in slow rolling when perfused at 100 s⁻¹_, 50 s⁻¹, and 10 s⁻¹ on immobilized VWF (HNA-3a, red, n = 6 donors per condition; HNA-3b, blue, n = 6 donors for 100 s⁻¹ and 5 donors for 50 s⁻¹ and 10 s⁻¹). (B-C) Percentage of total tracked neutrophils qualified as slow rolling (B) or rolling (C) when perfused at 100 s⁻¹ on immobilized VWF after LPS treatment (5 or 25 µg/mL) prior to perfusion (n = 5 donors per condition). (D-E) Percentage of total tracked neutrophils qualified as slow rolling (D) or rolling (E) when perfused at 100 s⁻¹ on immobilized VWF after treatment with a β₂ integrin blocking IgG or a control irrelevant IgG (HNA-3a, red, n = 4 donors; HNA-3b, blue, n = 3 donors per condition). Data are represented in nested form, values obtained for each field are shown, and 3 fields were analyzed for each experiment/donor (minimum/maximum, quartiles and medians are represented; nested t test; *P < .05; **P < .01).