Figure 1
Effect of HRG on the activated partial thromboplastin time in HRG-deficient plasma. HRG-deficient plasma was incubated with 0 to 6μM HRG for 10 minutes at 37°C. Clotting was initiated by the addition of CaCl2 to 26mM and one-tenth volume of APTT-SP. Absorbance was monitored at 405 nm, and the time to half-maximal value was defined as the clot time. Data are the mean ± SE of 3 determinations. The control was plasma subjected to chromatography on unmodified Sepharose.