Figure 2
Effect of HRG on clotting induced by FIXa, FXIa, or FXIIa in HRG-deficient plasma. HRG-deficient plasma was dialyzed to remove citrate and supplemented with 2mM CaCl2, 12.5μM ZnCl2, and increasing concentrations of HRG. Clotting at 37°C was initiated by the addition of 1.5nM FIXa (■), 0.5nM FXIa (
), or 5nM FXIIa (□) and monitored by turbidity. Clotting times are plotted versus HRG concentration. Data are mean ± SE of 3 determinations.