The WNT pathway regulates fibroblast activation. (A) Representative images of immunofluorescence staining for stress fibers, αSMA, and β-catenin in human dermal fibroblasts, with and without WNT inhibitors (original magnification ×200). Quantifications indicate that treatment with WNT inhibitors (24-hour incubation) prevents the upregulation of stress fiber formation, αSMA expression, and β-catenin accumulation in fibroblasts. (B) Representative pictures and quantifications of immunofluorescence staining for collagen type I and III in decellularized extracellular matrixes in human fibroblasts with or without WNT1 and with or without WNT inhibitors (48-hour incubation) (n = 4 cell lines each) (original magnification ×100). *P < .5; **P < .05; ***P < .01.