NSC12 mediates inhibition of MYD88 in WM cells. (A) BCWM.1 and MWCL.1 cells were exposed to NSC12 (6 μM) for 12 hours and subjected to wide transcriptome profiling, showing a significant inhibition of PI3K-AKT-mTOR- and MYD88-related gene sets, as shown by performing GSEA (P < .05; FDR < 25%). (B) BCWM.1 and MWCL.1 cells were cultured in the presence or absence of NSC12 (0 to 6 μM; 6 hours). WM cells were then harvested, and cell lysates were subjected to western blot using anti-MYD88, -p-SYK, -SYK, -p-BTK, -BTK, and -GAPDH antibodies. (C) BCWM.1 and MWCL.1 cells were treated with NSC12 (0 to 6 μM; 6 hours), subjected to RNA extraction, and evaluated for MYD88 and HCK mRNA levels by using qRT-PCR (2^−ΔΔCt), with normalization to GAPDH (P, P values). (D) BCWM.1 and MWCL.1 cells were cultured in the presence or absence of NSC12 (0 to 6 μM; 6 hours). WM cells were then harvested, and cell lysates were subjected to western blot using anti-c-Myc and -GAPDH antibodies.