Role of thrombin-driven fibrin polymer formation in liver injury after APAP overdose. Mice expressing mutant fibrinogen^AEK (Fib^AEK mice) and WT mice were treated intraperitoneally with saline vehicle of 300 mg/kg APAP and liver and citrated plasma were collected 24 hours after challenge. (A) Plasma ALT activity was determined using commercial reagents (n = 4 mice/group for vehicle treated and n = 11-12 mice/group for APAP challenged). (B) Area of centrilobular necrosis was quantified (n = 11-12 mice/group). (C) Representative photomicrographs of H&E-stained liver sections (necrosis denoted by black arrowhead). Data expressed as mean ± standard error of the mean. *Significantly (P < .05) different from vehicle-treated mice of the same genotype at 24 hours. ^#Significantly (P < .05) different from APAP-challenged WT mice.