P-selectin–deficient SCD mice exhibit amelioration of ischemic liver injury at baseline. (A) Schematic diagram of qLIM imaging of mice using TXR-dextran and CF to visualize blood flow and bile canaliculi, respectively. (B) qLIM images of 3 different FOVs of SS and P-selectin–deficient SCD liver (SS;Selp^−/−) injected with TXR-dextran and CF. Dotted circle shows loss of blood flow in SCD liver, which was significantly improved in SS;Selp^−/− liver. Original magnification, ×10; insets, ×20. (C) Quantification of the total area (%) of liver with loss of blood flow in SS and SS;Selp^−/− liver. (D) Percentage of total number of vasoocclusion/FOV. (E) Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in SS and SS-Selp^−/− mice. (F) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot analysis show comparable amount of HO-1 in SS and SS;Selp^−/− liver. *P < .05; **P < .01. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NS, not significant.