Figure 4.
HSCs from Thpo−/− mice exhibit attenuated oxidative stress upon romiplostim administration. (A) Representative plots and mean fluorescence intensity (MFI) of dendra2 fluorescence expressed in HSCs from mito-dendra2+/−;Thpo+/+ and mito-dendra2+/−;Thpo−/− mice, with or without administration of romiplostim (n = 5). (B) Ratio of mitochondrial DNA (mtDNA)/nuclear DNA (nucDNA) quantified by qPCR in Thpo+/+, Thpo−/−, Thpo+/+ Ro, and Thpo−/− Ro HSCs (n = 4). (C) ATP content measured in luminescence of Thpo+/+, Thpo−/−, Thpo+/+ Ro and Thpo−/− Ro HSCs (n > 5). Representative plots and MFI of CellROX staining (D) and ThiolTracker staining (E) in Thpo+/+, Thpo−/−, Thpo+/+ Ro, and Thpo−/− Ro HSCs (n = 5). (F) Representative flow cytometric plot, MFI, and percentage of positively gated cells based on Annexin V staining in Thpo+/+, Thpo−/−, Thpo+/+ Ro, and Thpo−/− Ro HSCs (n = 4). *P < .05, **P < .01, Student t test.

HSCs from Thpo−/− mice exhibit attenuated oxidative stress upon romiplostim administration. (A) Representative plots and mean fluorescence intensity (MFI) of dendra2 fluorescence expressed in HSCs from mito-dendra2+/−;Thpo+/+ and mito-dendra2+/−;Thpo−/− mice, with or without administration of romiplostim (n = 5). (B) Ratio of mitochondrial DNA (mtDNA)/nuclear DNA (nucDNA) quantified by qPCR in Thpo+/+, Thpo−/−, Thpo+/+ Ro, and Thpo−/− Ro HSCs (n = 4). (C) ATP content measured in luminescence of Thpo+/+, Thpo−/−, Thpo+/+ Ro and Thpo−/− Ro HSCs (n > 5). Representative plots and MFI of CellROX staining (D) and ThiolTracker staining (E) in Thpo+/+, Thpo−/−, Thpo+/+ Ro, and Thpo−/− Ro HSCs (n = 5). (F) Representative flow cytometric plot, MFI, and percentage of positively gated cells based on Annexin V staining in Thpo+/+, Thpo−/−, Thpo+/+ Ro, and Thpo−/− Ro HSCs (n = 4). *P < .05, **P < .01, Student t test.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal